Free Printable WorksheetScienceGrade 10Science CurriculumBIO.10.27

10.27 Diffusion and Facilitated Diffusion Worksheet

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Worksheet Overview & Skills Covered

Core academic competencies and curriculum alignment

This Biology Science worksheet provides structured practice on 10.27 Diffusion and Facilitated Diffusion. Students are guided through targeted exercises that build foundational understanding, computational accuracy, and critical thinking skills.

Diffusion and Facilitated Diffusion: Students predict movement of molecules down concentration gradients and identify how channel and carrier proteins enable facilitated diffusion. Rigor range: standard concept checks through honors/Pre-AP/AP-ready diagram, model, and stimulus-based cell or system analysis. Aligned to FreeK12Practice High School Biology Curriculum Crosswalk (NGSS/NRC Framework; CCSS Literacy and Mathematics; AP Biology enrichment; Texas TEKS Biology; Florida NGSSS/CPALMS Biology; Virginia SOL Biology; California NGSS and New York State Science Learning Standards) BIO.10.27 - Diffusion and Facilitated Diffusion: Corresponding standards copied from the curriculum for skill 10.27: National: NGSS HS-LS1-3; DCI LS1.A; Crosscutting Concepts: Cause and Effect; CCSS RST.9-10.7; HSS.ID.C.7; AP Biology ENE-2. State frameworks: TX TEKS BIO.5C; FL NGSSS SC.912.L.14.2; VA SOL BIO.3d; CA NGSS/NYSSLS HS-LS1-3.

Whether you are an educator preparing classroom handouts, a tutor reinforcing weekly topics, or a parent supporting homeschool learning, this printable resource gives students the deliberate practice required to achieve subject fluency and master state standards.

Grade

Biology

Subject

Science

Domain

Science Curriculum

Formats

PDF & DOCX

Sample Practice Problems

Preview the types of problems and questions covered in this worksheet

Below are sample questions representative of the exercises included on this worksheet and related 10.27 Diffusion and Facilitated Diffusion skill assessments:

Problem 1Difficulty: Mastery

A carrier transports Solute P down its gradient. When the carrier is removed, Solute P no longer crosses detectably, even though a steep gradient remains. When the carrier is restored, Solute P crosses until concentrations equalize. Which reasoning is strongest?

ASolute P crosses by simple diffusion because the gradient alone is sufficient

BThe carrier must be an ATP pump because equilibrium is reached

CSolute P is too small to interact with any membrane protein

DSolute P needs that carrier for facilitated diffusion, but the carrier does not move it beyond equilibrium

Solution & Explanation:The solute depends on the carrier to cross, yet movement stops at equal concentrations, showing passive facilitated diffusion rather than active pumping.

Problem 2Difficulty: Mastery

A model of a capillary wall shows oxygen, carbon dioxide, and glucose crossing into nearby cells. Oxygen and carbon dioxide cross the lipid bilayer, while glucose crosses through GLUT proteins. Which classification is correct?

AAll three use active transport pumps

BOxygen uses facilitated diffusion, while glucose uses simple diffusion

COxygen and carbon dioxide use simple diffusion; glucose uses facilitated diffusion

DOnly glucose can move down a concentration gradient

Solution & Explanation:Small nonpolar gases can diffuse through the bilayer, while glucose typically uses a carrier to diffuse down its gradient.

Problem 3Difficulty: Mastery

During a nerve cell response, a ligand-gated sodium channel opens. Sodium is more concentrated outside the neuron than inside. Which explanation best describes the sodium movement?

ASodium leaves the neuron by simple diffusion through the lipid bilayer

BSodium enters the neuron by facilitated diffusion through the open channel

CSodium enters the neuron only when ATP directly powers the channel

DSodium cannot move because ions are never transported across membranes

Solution & Explanation:The open channel provides a protein pathway, and sodium moves down its concentration gradient into the neuron.

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Frequently Asked Questions

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QWhat grade level and standards does this worksheet align with?

This worksheet is designed specifically for Biology Science students. It aligns with educational standard BIO.10.27 (Corresponding standards copied from the curriculum for skill 10.27: National: NGSS HS-LS1-3; DCI LS1.A; Crosscutting Concepts: Cause and Effect; CCSS RST.9-10.7; HSS.ID.C.7; AP Biology ENE-2. State frameworks: TX TEKS BIO.5C; FL NGSSS SC.912.L.14.2; VA SOL BIO.3d; CA NGSS/NYSSLS HS-LS1-3.).