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Evaluate Replication and Sample Size
Grade Level
AP Biology
APB.021 Evaluate Replication and Sample Size
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A Western blot compares insulin signaling using one treated cell lysate and one untreated lysate loaded in three lanes each. Which revision best improves reliability?
A
Load the same lysates in six lanes each without adding cultures.
B
Prepare lysates from several independent treated and untreated cell cultures.
C
Choose the band exposure that makes the treated lane darkest.
D
Use a different gel percentage for treated and untreated lysates.
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