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Temperature- or pH-Induced Denaturation
Grade Level
AP Biology
APB.168 Temperature- or pH-Induced Denaturation
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A researcher tests an enzyme after exposure to pH 7, pH 2, or 80 C. Only pH 2 and 80 C cause low activity after all samples are returned to pH 7 and 37 C. Which prediction is best supported?
A
pH 7 denatured the enzyme, while pH 2 and 80 C preserved the active site.
B
Extreme pH and high temperature disrupted enzyme structure in ways that persisted after normal conditions were restored.
C
The reaction depends only on final assay conditions, so pretreatment cannot matter.
D
Low activity must result from substrate loss because enzyme structure cannot persistently change.
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